Western Blot Positive WB detected in:Hela whole cell lysate treated by 15mM sodium butyrate for 30min All lanes:Acetyl-Histone H2B type 1-B(K20)antibody at 0.135μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 KDa Observed band size: 15 KDa
Immunocytochemistry analysis of CSB-RA010402A20acHU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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