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PODXL Monoclonal Antibody

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產(chǎn)品詳情相關(guān)產(chǎn)品

PODXL Monoclonal Antibody

貨號：

HYK-200031
規(guī)格：
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate
All lanes: PODXL antibody at 2.5μg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa

Western Blot
Positive WB detected in: A549 whole cell lysate
All lanes: PODXL antibody at 2.5μg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa

Western Blot
Positive WB detected in: Mouse brain tissue
All lanes: PODXL antibody at 0.43μg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa

Western Blot
Positive WB detected in: Rat brain tissue, Rat heart tissue, Rat kidney tissue
All lanes: PODXL antibody at 1.28μg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-MA134471A0m at dilution of 1:200

Immunohistochemistry of paraffin-embedded human melanoma using CSB-MA134471A0m at dilution of 1:200

Immunofluorescent analysis of Hela cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of PC-3 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of A549 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of Ntera-2 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Overlay histogram showing Hela cells stained with CSB-MA134471A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10μg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IGg2b (10μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

產(chǎn)品詳情

產(chǎn)品名稱：

Mouse anti-Homo sapiens (Human) PODXL Monoclonal antibody
Uniprot No.：

O00592
基因名：

PODXL
別名：

GCTM-2 antigen antibody; Gp2 antibody; Gp200 antibody; MGC138240 antibody; PC antibody; PCLP antibody; PCLP-1 antibody; PCLP1 antibody; Pcx antibody; Podocalyxin antibody; Podocalyxin like antibody; Podocalyxin like protein antibody; Podocalyxin-like protein 1 antibody; Podxl antibody; PODXL_HUMAN antibody
宿主：

Mouse
反應(yīng)種屬：

Human, Mouse, Rat
免疫原：

Recombinant Human PODXL protein
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

IgG2b
純化方式：

>95%, Protein G purified
克隆號：

4D2G7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, IP, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:500
IF	1:50-1:200
IP	1:200-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.